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The in Vitro Soluble RNA Methylase Activity of SV40-induced Hamster Tumors

Department of Viral Oncology, General Clinical Research Center, and the Department of Experimental Therapeutics, Roswell Park Memorial Institute, Buffalo, New York 14203

The in vitro methylase activity of SV40-induced hamster tumors and normal hamster tissues has been studied. Extracts of normal hamster tissues such as connective tissue, liver, thymus, and skeletal muscle are capable of hypermethylating yeast soluble RNA (sRNA) in the N¹ position of guanosine and possibly of adenosine. Extracts of SV40-induced tumors are capable of hypermethylating yeast sRNA more extensively. Incorporation of radioactive methyl-¹⁴C groups was found in N¹-methylguanosine, N¹, N¹-dimethylguanosine, N⁶-methyladenine, and 5methyluridine.

SV40 tumor extracts are capable of hypermethylating homologous sRNA extracted from normal hamster liver while extracts of normal tissues cannot. Significant incorporation of methyl-¹⁴C groups was found in the N¹-methylguanosine of hamster sRNA hydrolysates.

Received 8/22/66. Accepted 4/ 7/67.