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Amino Acids of the Ornithine Cycle in Transformed Hamster Fibroblasts Carrying Pleuropneumonia-like Organisms¹

Stanford Research Institute, Life Sciences Area, Menlo Park, California 94025

The profile of free amino acids was analyzed in normal embryonic hamster cell cultures, in two types of transformed fibroblasts of similar origin (one transformed by polyoma virus and the other spontaneously), in the tumors grown from each transformed type by implantation into hamsters, and in the cell cultures of the respective tumors. Both types of transformed cells, and their respective tumors and tumor-cell cultures, had very low concentrations of arginine and high concentrations of citrulline when compared with the levels of these amino acids in normal embryonic cell cultures. There were no consistent differences among free amino acids between the two types of transformed hamster fibroblasts, viral and spontaneous. A random virus-transformed culture carried pleuropneumonia-like organisms.

¹ This work was supported in part by Contract PH 43-65-43 from the Cancer Chemotherapy National Service Center, National Cancer Institute, NIH.

Received 1/ 5/67. Accepted 4/20/67.