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[Cancer Research 27, 1507-1512, September 1, 1967]
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Distribution Studies of Tryptophan Mustard-3H in Normal and Tumor-bearing Rats1

Ralph K. Barclay, Tadashi Yoshida2, Mildred A. Phillipps, Olga Terebus-Kekish and Marion Barclay

Division of Experimental Chemotherapy, Sloan-Kettering Institute for Cancer Research; and Sloan-Kettering Division, Graduate School of Medical Sciences, Cornell University Medical College, New York, New York 10021

Tritiated 5-bis(2-chloroethyl)amino-DL-tryptophan has been synthesized, and distribution of radioactivity in rats bearing Jensen sarcoma has been determined. Three hr after i.p. injection (5 mg/kg, 0.2 µc/mg), 35% of the total activity was measured in liver, 14% in blood, 12% in kidney, and 6% in spleen. Kidney had the highest specific activity, followed by spleen, liver, heart, blood, small intestine, adipose tissue, and tumor. The highest activity in liver, spleen, and blood occurred 15 min or less after injection and decreased rapidly, while activity in kidney increased with time. In the 3 most active tissues, incorporation of radioactivity was highest in protein (60–80%), intermediate in lipid (11–15%), and low but significant in nucleic acids (3–5%). Fractionation of serum into known lipoprotein components revealed highest activity in high density lipoproteins ("ultracentrifugal residue" and HDL2) within 15 min; by 3 hr most of the activity was in the residue fraction. Lipoprotein fractions from serum from tumor-bearing rats contained more radioactivity than from normal rats.

1 This investigation was supported by Grants CY-3192 and CA 08748 from the National Cancer Institute.

2 Present address: Shionogi Research Laboratories, Shionogi and Co., Ltd., Osaka, Japan.

Received 8/ 5/66. Accepted 4/27/67.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cancer Prevention Research
Cancer Prevention Journals Portal Cancer Reviews Online
Annual Meeting Education Book Cell Growth & Differentiation
Copyright © 1967 by the American Association for Cancer Research.