This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Mayfield, E. D. Articles by Bresnick, E. Search for Related Content PubMed PubMed Citation Articles by Mayfield, E. D., Jr. Articles by Bresnick, E.

Activities of Enzymes of Deoxyribonucleic Acid Synthesis After Unilateral Nephrectomy¹

Departments of Pharmacology and Anatomy, Baylor University College of Medicine, Houston, Texas 77025

The biosynthesis of DNA and the activities of enzymes involved in this pathway were investigated in the surviving rat kidney after unilateral nephrectomy. It was shown by radioautographic technics that DNA synthesis is markedly enhanced by 24 hours and reaches a peak at 48 to 72 hours postoperatively. Deoxythymidine (thymidine; thymine deoxyribonucleoside) kinase, deoxythymidine monophosphate kinase, and 2'-deoxycytidine-5'-monophosphate (dCMP) deaminase also increased in activity, reaching a maximum at 24 to 48 hours postoperatively, whereas 2'-deoxythymidine-5'-monophosphate phosphatase and dCMP kinase did not show any consistent change. These changes were observed just prior to the burst in mitotic activity as measured by radioautographic technics.

¹ This investigation was supported by a grant from the USPHS, CA-06571, and by an institutional grant from the American Cancer Society.

² Recipient of a Lederle Medical Faculty Award. Inquiries should be addressed to Dr. Bresnick.

Received 2/ 9/67. Accepted 5/12/67.