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The Cellular Analysis of Liver Carcinogenesis: Ultrastructural Alterations within Hyperplastic Liver Nodules Induced by 2-Fluorenylacetamide¹

The William H. Singer Memorial Research Institute of Allegheny General Hospital, and Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania

The fine structure of 2-fluorenylacetamide-induced hyperplastic liver nodules and non-nodular surrounding liver in rats following a 24-hour fast was described. Within the nodule, annulate lamellae were frequently observed. These organelles were associated with an increase in smooth endoplasmic reticulum frequently arranged in cluster formation. In addition, within the nodule there was a concomitant decrease in parallel-arrayed rough endoplasmic reticulum and numerous small, lightly staining vacuoles. On the other hand, the adjacent non-nodular liver did not contain annulate lamellae, but did show large osmiophilic lipid bodies. Surrounding liver displayed preservation of rough endoplasmic reticulum and less clustered smooth endoplasmic reticulum. The combination of ultrastructural features imparted a characteristic appearance which specifically differentiated the hyperplastic nodule from adjacent liver.

¹ Presented in part at the Sixth Annual Meeting of the American Society for Cell Biology, Houston, Texas, November, 1966.

² This research was supported in part by grants from the American Cancer Society and the Beaver County Cancer Society and by the USPHS Research Grants CA-06074 from the National Cancer Institute and GM-135 (a training grant) and GM-10269 (a research grant) from the National Institute of General Medical Sciences and by a contract (PH-436-4505) from the National Cancer Institute.

Received 2/23/67. Accepted 5/16/67.

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