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A Method for the Experimental Induction of Bronchogenic Carcinoma¹

Division of Oncology, Institute for Medical Research, The Chicago Medical School, Chicago, Illinois 60612

A methodology for the experimental induction of bronchogenic carcinoma in conditions related to those of human exposure to respiratory carcinogens is described. Animal of choice is the hamster. Polynuclear hydrocarbon carcinogens are prepared as suspensions of fine crystalline particles and attached to a finely particulated inert dust which acts as a carrier; the mixed dust is suspended in saline and administered by intratracheal instillation. Penetration and distribution patterns are described. No necrosis and no chronic inflammatory reactions other than phagocytosis are induced. Repeated intratracheal instillations of benzo[a]pyrene with hematite dust induce up to 100% incidences of respiratory tract tumors, mostly bronchogenic carcinomas. The morphology of these tumors appears very close to that of human lung cancer; squamous cell carcinomas are the most frequent type followed by anaplastic carcinomas and by a few adenocarcinomas. The experimental model presented here appears adequate for studies on factors involved in the pathogenesis of cancer of the lung.

¹ Supported by Grant No. E-214 from the American Cancer Society and by Research Project Contract No. MC-6 from the American Petroleum Institute. Parts of this work were presented at the 54th and 55th Annual Meetings of the American Association for Cancer Research held respectively in Toronto, Ont., May, 1963 and in Chicago, Ill., April, 1964; part of the iconography was shown in an exhibit at the Third Quadrennial Internat. Conference on Cancer, "Lung Tumors in Animals," Univ. of Perugia, Italy, June, 1965.

² Recipient of USPHS Research Career Development Award K3-CA-25,027 from the National Cancer Institute, NIH.

³ Trainee of USPHS Graduate Training Grant No. CA-5070. Present address: Institute of Pathologic Anatomy, University of Milan, Italy.

Received 2/16/67. Accepted 9/25/67.

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