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DNA Metabolism in Liver and Kidney Tumors of Different Growth Rates¹

Department of Pharmacology, Indiana University School of Medicine, Indianapolis, Indiana (M.A.L., G.W.), and Laboratory of Biochemistry, National Cancer Institute, NIH, USPHS, Bethesda, Maryland (H.P.M.)

The incorporation of ¹⁴C-labeled thymidine into DNA was studied in liver and kidney tumors of different growth rates. Low incorporation was observed for the normal kidney cortex whereas greatly increased rates were found even in the very slowly growing 8997-K renal cortical tumor. The largest incorporation was observed in the most rapidly growing of the kidney tumors examined, the 9789-K. A similar trend was observed in the neoplastic liver, confirming our previous report. The ratio of thymidine to deoxyuridine incorporation into DNA was approximately 5 in control livers and was greater in regenerating liver and in the three hepatomas examined. Deoxyuridine incorporation into DNA was increased in the regenerating liver and the rapidly growing 3924-A and 3683 hepatomas but not in the slowly growing 7794-B hepatoma. The rate of dilution of ¹⁴C-labeled DNA in vivo was examined in control liver and three hepatomas. It was concluded that this procedure may serve as an alternative measure to thymidine incorporation in assessing the rate of DNA synthesis of these tumors.

¹ This paper is Number 7 in a series on Comparative Biochemistry of Hepatomas. This investigation was supported by grants from the USPHS (CA-05034), the American Cancer Society, the Damon Runyon Memorial Fund, Inc. and the American Cancer Society Institutional Grant.

Received 6/ 5/67. Accepted 9/26/67.