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Electron Microscopy of a Herpes Virus Associated with the Agent of Marek's Disease in Cell Culture

United States Department of Agriculture, Poultry Research Branch, Animal Husbandry Research Division, ARS, Regional Poultry Research Laboratory, East Lansing, Michigan 48823

Duck embryo fibroblast cultures inoculated with blood from chickens infected with JM strain of Marek's disease developed a cytopathic effect 11–25 days postinoculation. DNA containing intranuclear inclusion bodies were seen in many polykaryocytes associated with the cytopathic effect.

Some intranuclear granular bodies were seen in thin sections of these cells examined with the electron microscope. The granular bodies probably corresponded to the intranuclear inclusion bodies seen with the light microscope. Naked herpes virus-like particles and occasional particles with an additional membrane were found in the nuclei of infected cells. The naked particles appeared to start budding into the cytoplasm and nuclear vesicles and obtain an additional membrane in this process. The naked and enveloped particles were occasionally seen in the cytoplasm accompanied with some membrane-like structures. However, only typical naked particles were seen in negatively stained preparations of concentrated culture fluids. These particles measured 95–100 mµ and had 162 capsomeres. The morphogenesis and morphology of the virus were similar to those of herpes-type viruses.

Received 3/21/68. Accepted 8/ 3/68.

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