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The Antigenicity and Immunogenicity of Cell-free Extracts of Chemically Induced Murine Sarcomas

Surgery Branch, National Cancer Institute, NIH, Bethesda, Maryland 20014

The induction of antitumor isoimmunity to an ethiodolinduced fibrosarcoma in female BALB/c mice and to a methylcholanthrene-induced fibrosarcoma (MC 1) in female C3H/HeN mice was accomplished using three different cell-free extracts of tumor tissue, a saline extract and two different fluorocarbon-treated extracts. The degree of immunity observed was dependent upon the length and intensiveness of the course of immunization, the route of immunization, and the size of the challenge tumor-cell inoculum. The presence of a circulating antibody of low titer was detected in immunized animals exhibiting significant tumor immunity. This antibody was not demonstrable in control animals or in immunized animals in which no significant immunity was observed. Animals immunized with extracts of normal BALB/c or C3H/HeN tissues did not exhibit increased resistance to tumor transplants. BALB/c mice rendered immune to one ethiodol-induced sarcoma displayed no increased resistance to transplants of another ethiodol-induced sarcoma arising in another mouse of the same strain. C3H/HeN mice immune to challenge with MC 1 cells exhibited no increased resistance to transplants of a breast carcinoma (Sp 1) which arose in a mouse of the same strain. Antibodies detected to the MC 1 tumor in C3H/HeN mice did not cross-react with antigens from the Sp 1 tumor. Both the immunity produced and the antibodies detected were shown to persist for at least 4.5 months.

Received 9/ 8/67. Accepted 8/ 7/68.

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