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Patterns of Three-dimensional Growth in Vitro in Collagen-coated Cellulose Sponge: Carcinomas and Embryonic Tissues¹

Department of Pathology, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15213

Several transplantable tumors and chick embryonic tissues were cultured in collagen-coated cellulose sponge. The growth obtained in this matrix was compared with that obtained in cellulose sponge alone and in collagen sponge alone. The tumors included a mouse mammary adenocarcinoma (C3HBA), a squamous cell carcinoma of the mouse cervix (D-98), a mouse ascites hepatoma (Sato A H #134), a rat ascites hepatoma (Yoshida #7974), and a human adenocarcinoma of the colon (H.Ad.-1). The embryonic tissues were intestine, lung, and heart. All the sponges were examined in histologic section. The best patterns of migratory outgrowth were seen in collagen-coated cellulose sponge. Conventional histologic criteria were used to identify the cellular components of the newly formed tissues.

¹ This investigation was supported by research Grant P-422 from the American Cancer Society and by research Grant 950 from the Damon Runyon Memorial Fund. The work was also aided by Grants GM 10269 (research grant) and GM 135 (training grant) from the National Institute of General Medical Science.

Received 8/16/67. Accepted 10/11/67.