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Cellular Antigen of Friend Virus-induced Leukemias¹

Department of Biology, McMaster University, Hamilton, Ontario, Canada

In a study of the components of antiserum prepared (in SIM/McK mice) against formalinized Friend leukemic cell suspensions, the spleen focus assay method was employed for measuring the kinetics of virus neutralization, and the spleen colony method was used to monitor the loss of tumor colony-forming ability during the cytotoxic reaction.

Repeated absorption of Friend antiserum with resuspended pellets of Friend virus harvested from plasma revealed that most of the virus-neutralizing activity of the antiserum could be removed without altering its cytotoxic activity. Absorption with plasma pellets from uninfected mice did not alter either activity.

It is concluded that the "cellular" antigen involved in the cytotoxic reaction is distinct from the antigen involved in virus neutralization.

¹ This research was supported by the National Cancer Institute of Canada.

² Present address: Department of Viral Oncology, Roswell Park Memorial Institute, Buffalo, New York 14203.

Received 7/17/67. Accepted 10/17/67.