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A Comparison of the Distribution of Tumors Produced by Intravenous Injection of Type 12 Adenovirus and Adeno-12 Tumor Cells¹

Section of Viral Oncology and the Department of Experimental Pathology, Roswell Park Memorial Institute, and Department of Oral Pathology, School of Dentistry, State University of New York at Buffalo, Buffalo, New York 14203

Intravenous inoculation of 10⁶ adeno-12 tumor cells in 19 newborn hamsters resulted in multiple tumors distributed in 100% of the livers, kidneys, lungs, and adrenals. All but one heart contained tumors. Gonads, spleen, and pancreas also supported tumor growth but in less than half the animals. Tumors were even fewer in the intestine, thymus, muscles, bone marrow, and brain; in the latter three areas only a single tumor was found. Tumors were not distributed according to organ weight; their distribution appeared to be influenced by anatomic and physiologic factors.

Intravenous inoculation of 10⁸ adenovirus-12 TCD₅₀ in 16 newborn hamsters resulted in tumors in 100% of the livers and 38% of the kidneys. Two tumors were found in the intestines and at the injection site. One tumor each developed in the lungs, pancreas, and skeletal muscle of different hamsters. Radioactive (³H) virus was relatively evenly distributed to all body organs, as determined by radioautography. Nonetheless, certain sites which had been shown to support tumor growth and which received sufficient virus remained free of virus-induced tumor. It was concluded that the liver and kidneys contained particularly susceptible "target" cells and a favorable environment for tumor growth. The heart and lungs, although presenting a presumably favorable environment, either contained insufficient "target" cells or received an insufficient virus dose.

Discussion of the distribution of tumors induced with adenovirus-12 in hamsters included a comparison with the polyoma model, a system in which the virus is known to replicate in the host as contrasted to the adenovirus model.

¹ Supported in part by research grant CA-07745 from the USPHS, a grant from the John A. Hartford Foundation, and Grant P-403A from the American Cancer Society.

Received 8/17/67. Accepted 11/29/67.