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Transfer and Polymerization of Amino Acids from Aminoacyl Ribonucleic Acids with a Tumor Ribosomal System¹

Department of Biochemistry, The University of Texas, M. D. Anderson Hospital and Tumor Institute at Houston, Houston, Texas 77025

Components involved in the transfer of aminoacyl transfer ribonucleic acid to the messenger polysomal complex and in amino acid polymerization have been isolated from Novikoff ascites tumor cells and from normal liver. The components of the two systems are completely interchangeable. A partial purification of the tumor cell transfer enzyme complex was achieved; however, the complex has not been resolved into two or more enzymes as reported for other incorporating systems. The reconstituted tumor system is highly active in terms of amino acid incorporation. It is dependent upon guanosine triphosphate and transfer enzymes. Ammonium ions are the most effective as a source of monovalent cation while potassium and sodium are active to a lesser extent. Incorporation is inhibited by cycloheximide but not by the addition of chloramphenicol.

¹ This work was supported by grants from the American Cancer Society and The Robert A. Welch Foundation.

² American Cancer Society Professor of Biochemistry.

Received 1/30/67. Accepted 12/ 1/67.