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The Rapid Collection and Application of Cigarette Smoke Condensate¹

Department of Physical and Biological Sciences, Southwest Research Institute, San Antonio, Texas 78206

A practical system for the collection and application of cigarette smoke is described. The apparatus is judged to be easier to operate and maintain than systems employing cold traps or solvent liquid traps. Condensation of the smoke is accomplished through the use of a restricting nozzle. Application of the condensate may be an indirect or direct operation. In the indirect mode of operation, a weighing bottle is used to collect the condensate which may then be used for analytic or biologic test purposes; in the direct mode of operation, the smoke is condensed on the shaved skin of a mouse. Thus, the system permits the use of condensates which have existed only seconds or minutes in contrast to the well-aged condensates used by most investigators. The freshness of the condensates used and the conditions under which they are obtained may be important with respect to the conditions which prevail in human smoking. Animal tests are in progress which employ the technics described herein.

¹ The work reported has been conducted under Contract No. PH43-65-1000 for the Carcinogenesis Studies Branch, National Cancer Institute, NIH, Bethesda, Maryland.

Received 9/25/67. Accepted 1/17/68.