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Determination of Certain Antitumor Agents in Mouse Blood by Microbiologic Assay¹

Kettering-Meyer Laboratories, Southern Research Institute,² Birmingham, Alabama 35205

Microbiologic assays have been adapted or developed for the estimation of certain antitumor compounds (or cytotoxic equivalents) in biologic tissues. Sufficiently sensitive assay procedures for measuring blood concentrations following realistic therapeutic doses in mice are described for 6-methylmercaptopurine riboside (NSC 40774), 5-fluorouracil (NSC 19893), 5-fluorodeoxyuridine (NSC 27640), Methotrexate (NSC 740), and actinobolin (NSC 31083). Less sensitive, but sometimes useful, assay procedures are described for 6-mercaptopurine (NSC 755), 6-mercaptopurine riboside (NSC 4911), and 2-chloro-4',4''-di-2-imidazolin-2-yl, terephthalanilide (NSC 60339). Assay procedures for actinomycin D (NSC 3053) and 1,3-bis(2-chloroethyl)-1-nitrosourea [BCNU] (NSC 409962) are presented; however, the latter two assays lack sufficient sensitivity to measure nonlethal doses of drug.

¹ This work was supported by the Cancer Chemotherapy National Service Center, National Cancer Institute under NIH Contract Number PH43-65-594.

² Affiliated with Sloan-Kettering Institute for Cancer Research, New York.

Received 11/ 6/67. Accepted 2/19/68.