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The Histopathology and Ultrastructure of Tumors Induced by Simian Adenovirus 30^{1 ,2}

Departments of Experimental Pathology and Microbiology, William H. Singer Memorial Research Institute of the Allegheny General Hospital, and Department of Microbiology, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15212

Cesium chloride-purified preparations of simian adenovirus 30 (SV30) were found to be oncogenic when inoculated into newborn hamsters. Subcutaneous tumors developed in low incidence (<2%), and a histopathologic pattern similar to that induced by oncogenic human adenoviruses (25, 37, 51) was described. The neoplasm induced by SV30 and the biologic activity of the virus appear to have notable differences from that reported for SV40 and its corresponding neoplasm. The ultrastructure of these malignant cells revealed them to have relatively undifferentiated cytoplasmic organelles. Annulate lamellae and virus-like particles with an average diameter of 80 mµ were observed in neoplastic cells. These particles within tumor cells resembled the morphologic appearance characteristic of infectious simian adenoviruses and displayed "budding" from the endoplasmic reticulum. Speculative implications of the presence of virus-like particles in SV30-induced tumor cells are discussed.

¹ Presented in part at the Seventh Annual Meeting of the American Society for Cell Biology, Denver, Colorado, November, 1967.

² This investigation was supported in part by Grant AI-02535 from the NIH.

³ Present Address: Division of Biological Research, G. D. Searle and Company, Chicago, Illinois 60680.

Received 10/13/67. Accepted 2/28/68.