This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kurita, Y. Articles by Nishizuka, Y. Search for Related Content PubMed PubMed Citation Articles by Kurita, Y. Articles by Nishizuka, Y.

Cytogenetic Studies on Rat Leukemia Induced by Pulse Doses of 7,12-Dimethylbenz()anthracene¹

Laboratory of Experimental Pathology, Aichi Cancer Center Research Institute, Chikusa-ku, Nagoya, Japan

Cytogenetic studies were carried out on bone marrow and spleen cells of 40 rats of Long-Evans strain with primary leukemia induced by pulse doses of 7,12-dimethylbenz()anthracene. Chromosomal abnormalities were detected in 26 cases, of which 21 were characterized by abnormal constitution of two specific chromosomes, C-1 (longest telocentric chromosome), and A-6 (6th group of metacentrics). Most of the leukemias represented the hepatic type. Sixteen rats had modal or nonmodal cells with trisomic C-1 chromosome, 5 among them containing stem-line cells with both C-1 and A-6 trisomy. Unassociated A-6 trisomy was found in modal cells of 4 other leukemias, one of them chloroleukemia, and in the stemline of one of the trisomic C-1 positive leukemia. Modal hypodiploid cells of 41 chromosomes with A-6 monosomy was also detected in one case. These consistent chromosomal abnormalities appear to be the first so far reported for an experimental leukemia. The presence of a marker chromosome, metacentric or submetacentric, was recognized in cells of 4 other leukemias. The remaining one had tetraploid cells. No chromosomal anomaly could be detected in 12 other hepatic type leukemias, one lymphoblastic and another thymic. The possible interpretation for the presence of consistent chromosomal abnormalities in the given type of leukemia is discussed.

¹ This study was partly supported by a Grant-in-Aid for Fundamental Scientific Research from the Ministry of Education.

Received 12/26/67. Accepted 5/13/68.

This article has been cited by other articles:

				F. Mitelman, J. Mark, G. Levan, and A. Levan Tumor Etiology and Chromosome Pattern Science, June 23, 1972; 176(4041): 1340 - 1341. [Abstract] [PDF]