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Immunofluorescence and Virus Recovery: Correlation in a Murine Leukemia System¹

Bionetics Research Laboratories, Inc., Kensington, Maryland

Results from mouse infectivity assays and immunofluorescent examinations during pathogenesis of the Moloney murine leukemia virus infection in adult BALB/c mice indicated that the two detection methods could be only partially correlated. The fluorescent antibody technic detected viral antigens as early as 4–8 days after virus inoculation, although infectious virus could not be extracted from the same tissues until the 16th day of the infection. In terms of infectivity titers, immunofluorescence detected less than one-half the log of a 50% infectious dose of virus. Cells from either spleen or peripheral blood of inoculated mice reacted identically in the fluorescent antibody tests.

¹ Submitted in part to The George Washington University in partial satisfaction of the requirements for the degree of Doctor of Philosophy.

² Present address: Laboratory of Virology & Rickettsiology, Division of Biologics Standards, National Institutes of Health, Bethesda, Md.

³ Chief, Viral Leukemia and Lymphoma Branch, National Cancer Institute, National Institutes of Health, Bethesda, Md.

Received 1/ 2/68. Accepted 5/14/68.