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Concentration by Diaflo Ultrafiltration of Murine Leukemia and Sarcoma Viruses Grown in Tissue Cultures^{1 ,2}

Department of Virus Research, Microbiological Associates, Inc., and Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20014

Ultrafiltration by Diaflo membranes provides a simple and useful method for achieving quantitative concentration of murine leukemia and sarcoma viruses and also group-specific complement-fixing antigens shared in common by this group of agents.

¹ Supported by Contract PH 43-62-200 of the NIH, National Institute of Allergy and Infectious Diseases.

² A preliminary report of this work was presented at the Fifty-second Annual Meeting of the Federation of American Societies for Experimental Biology and Medicine, Atlantic City, New Jersey, April 15–20, 1968. (Federation Proc., 27: 734, 1968)

Received 5/20/68. Accepted 8/28/68.