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Carcinogenesis in Rats by Aflatoxins B₁, G₁, and B₂¹

Division of Oncology, Institute for Medical Research, The Chicago Medical School, Chicago, Illinois 60012, Toxicology Research Unit, Medical Research Council, Carshalton, Surrey, England, and Eppley Institute for Research in Cancer, University of Nebraska, College of Medicine, Omaha, Nebraska 68105

Aflatoxins B₁, G₁, and B₂ of high purity have been prepared from a crude mixture of aflatoxins and have been tested by long-term feeding in drinking water to rats, at concentrations of 1 µg/ml and 3 µg/ml. Aflatoxin B₁ produced liver tumors in 19 of 30 rats given a total dose of 2 mg each; 3 of 10 animals receiving a total dose of 1 mg developed liver tumors. Aflatoxin G₁ gave rise to liver tumors in 3 of 30 animals given a total dose of 2 mg and to 1 liver tumor in 10 animals given 1 mg each. However, of 26 rats receiving a total of 6 mg aflatoxin G₁, 21 animals developed liver tumors, and 6 animals had kidney tumors. A number of animals receiving 2 mg of aflatoxin G₁ also had kidney tumors. There was no sex difference in the incidence of liver tumors, but kidney tumors were seen only in males. The liver tumors were almost all hepatocellular carcinomas. No liver tumors were seen in 10 rats receiving a total dose of 1 mg aflatoxin B₂.

¹ Supported by USPHS Contract No. 43-65-67 and 43-68-959 from the National Cancer Institute.

² Present address: Toxicology Research Unit, Medical Research Council Laboratories, Carshalton, Surrey, England.

³ Present address: Eppley Institute for Research in Cancer, University of Nebraska College of Medicine, Omaha, Nebraska 68105.

⁴ Recipient of a Research Career Development Award, USPHS.

Received 2/ 3/69. Accepted 4/24/69.