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The Effect of Nucleoproteins upon Amino Acid Incorporation into Protein in Walker 256 Cells¹

The Departments of Chemistry and Medicine, The University of California, Los Angeles, Los Angeles, California 90024

The effects of deoxyribonucleoprotein, histones, and poly-L-lysine on amino acid uptake and incorporation into protein in Walker 256 tumor cells have been investigated. When Walker 256 tumor cells were incubated with deoxyribonucleoprotein, histones, or poly-L-lysine there was a reduction in the amount of ¹⁴C-labeled amino acids found in the hot 5% trichloroacetic acid-insoluble fraction. The very lysine-rich histone fractions produced the greatest percent of inhibition. Homologous histones at low concentrations were strongly inhibitory while homologous deoxyribonucleoproteins gave slightly less inhibition.

The inhibition produced by deoxyribonucleoprotein could be partially removed by washing the cells with cold incubation medium. However, the inhibition caused by histones remained even after washing with cold incubation medium.

These findings suggest that it is the histone portion of the nucleoprotein that is responsible for the observed inhibition of protein elaboration and that the effect is either an intracellular or membrane phenomenon.

¹ This work was supported by Grants CA 10308 from the USPHS and by The Julius and Dorothy Fried Research Foundation.

Received 12/13/68. Accepted 4/30/69.