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Effect of an Extract of UV-irradiated Linolenic Acid on Azo Dye Carcinogenesis

National Cancer Institute, Bethesda, Maryland 20014, and Armed Forces Institute of Pathology, Washington, D. C. 20305

One to four intraperitoneal injections of water extract of UV-irradiated linolenic acid were given to male Osborne-Mendel rats. Injections were at monthly intervals with graded doses (120 to 600 thiobarbituric acid units/kg/dose) of the extract. Some of these rats received a subcarcinogenic dose (0.12%) of p-dimethylaminoazobenzene (DAB) in powdered Purina chow diet from the day of first injection for four months. The experiments were terminated two months later. Four hundred units or more of UV-extract alone were lethal within 72 hours, lower dosage of the UV-extract produced chronic toxicity, with swelling of the liver, fusion of its lobes, and adhesions to surrounding structures. DAB in the diet for 4 months and 3 or 4 injections of UV-extract caused extensive cholangeofibrosis of the liver in all the animals and hepatoma nodules in 25% of the rats. Activity of some oxidases, dehydrogenases, and phosphatases of the livers of these rats was 25 to 40% lower than that of the comparable enzymes in control rats.

¹ Present address: National Bureau of Standards, Gaithersburg, Maryland 20760.

² Present address: Microbiological Associates, Inc., Bethesda, Maryland 20014.

Received 6/13/68. Accepted 9/28/68.