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The Effect of Phleomycin on the Replication of Papovavirus SV40 and Other DNA Viruses in Simian Cells¹

Department of Virology and Epidemiology, Baylor University College of Medicine, Houston, Texas 77025

Phleomycin, a copper-containing antibiotic, was effective in blocking the synthesis of infectious simian papovavirus SV40. Maximum effect of the antibiotic was evident between 12 and 30 hours following the inoculation of the cultures with SV40, a time previously shown to represent the height of viral DNA synthesis. Synthesis of SV40 viral antigen was markedly reduced when the cultures were maintained in the presence of Phleomycin. Phleomycin had no effect on the synthesis of SV40 tumor antigen. The antibiotic was effective in inhibiting vaccinia virus multiplication but less effective against members of the herpes virus group. Synthesis of SV15, a simian adenovirus, was inhibited moderately. The differential effect of Phleomycin seems to depend upon the content of adenine and thymine in the viral DNA. Phleomycin did not affect the replication of the RNA-containing poliovirus in the same cells.

¹ Supported in part by research Grant CA 10036 from the National Cancer Institute, NIH.

² Recipient of Research Career Development Award No. 1-K3-CA38,614 from the National Cancer Institute, NIH.

³ American Cancer Society Professor of Virology.

Received 8/12/68. Accepted 11/27/68.