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Changes in DNA-bound Amino Acids in Experimental Tumor Transplants^{1 ,2}

Sloan-Kettering Institute for Cancer Research and the Sloan-Kettering Division Graduate School of Medical Sciences, Cornell University Medical College, New York, New York 10021

The DNA-bound amino acids have been determined in a number of primary tumors: one virus induced, two spontaneous, and several chemically induced. The total amounts in tumors are larger than normal tissues. The relative amounts are, in some cases, identical with those of the tissues of origin. In most, but not all, primary tumors an unusual amino acid component not found in cellular protein hydrolysates was eluted from the ion-exchange column at the same time as the aromatic amino acids. In general, this new material persists through several transplant generations but, ultimately, seems to disappear.

One tumor was examined through twenty-five transplant generations. There were variations in the total amino acid components of the DNA and in the individual amino acids, particularly the unusual one. These differences were generally found to coincide with morphologic changes, suggesting that they may be interrelated.

¹ A preliminary report of part of this work has been presented (11).

² This investigation was supported in part by funds from USPHS Grant No. CA-08748 and the Damon Runyon Memorial Fund for Cancer Research, Grant No. 937.

Received 8/15/68. Accepted 12/10/68.