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Comparison of Transfer Ribonucleic Acids and Aminoacyl Synthetases of Liver and Ascites Tumor Cells¹

Department of Biochemistry, The University of Texas M. D. Anderson Hospital and Tumor Institute at Houston, Houston, Texas 77025

Aminoacyl synthetase and transfer ribonucleic acid (tRNA) fractions were obtained from normal rat liver and from Novikoff ascites tumor cells. The tRNA was precharged with tritium and ¹⁴C-labeled arginine, phenylalanine, or valine employing various combinations of the synthetase and tRNA fractions. The labeled aminoacyl tRNA's were resolved employing reversed phase chromatography. The tumor and liver cell patterns for arginyl tRNA were almost identical, while the cell types differed with respect to the phenylalanyl tRNA profile. When liver phenylalanyl tRNA was chromatographed on the reversed phase columns, three distinct peaks were obtained. In contrast, the tumor phenylalanyl tRNA did not exhibit the first peak seen in the liver profile, and the second tumor phenylalanyl tRNA peak was displaced.

¹ Supported by American Cancer Society Grant E-312 and The Robert A. Welch Foundation Grant G-035.

² American Cancer Society Professor of Biochemistry.

Received 6/17/68. Accepted 12/20/68.