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A Microsome-dependent Binding of Benzo[a]pyrene to DNA¹

Chemistry Branch, National Cancer Institute, NIH, Bethesda, Maryland 20014

Benzo[a]pyrene-³H incubated in the presence of rat liver microsomes with calf thymus DNA binds covalently to the DNA. The reaction requires reduced nicotinamide adenine dinucleotide phosphate and is greater when the microsomes are obtained from rats pretreated with polycyclic hydrocarbons. The binding is due to the formation of unknown metabolite(s) which then may bind to the DNA in the absence of microsomes.

¹ A report of this work was presented at the International Symposium on Physicochemical Mechanisms of Carcinogenesis, Israel Academy of Sciences and Humanities, Jerusalem, Israel, October 1968.

Received 10/28/68. Accepted 2/ 2/69.

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