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Apparent Antimutagenic Activity of Quinacrine Hydrochloride in Detroit-98 Human Sternal Marrow Cells Grown in Culture

The Upjohn Company, Research Division, Kalamazoo, Michigan 49001

The ability of a small proportion of the cells in cultures of Detroit-98 (D-98) human sternal marrow cells to attach to glass in the presence of 8-azaguanine has been previously shown to be caused by a mutation to resistance to this compound. This paper presents results of experiments dealing with the effect of the addition of quinacrine hydrochloride to the culture medium of D-98 cells on the frequency of this mutation. Such cells have been previously shown to be azaguanine-resistant mutants. In three independent experiments, cultures which were grown for several subcultivations in the presence of 0.06 µg/ml of quinacrine hydrochloride contained one-half to one-third the mean number of azaguanine-resistant mutants found in control cultures. On the other hand, the incorporation of quinacrine hydrochloride into the culture medium of D-98/AG cells, an azaguanine-resistant subline of the D-98 line, had no effect on its cloning efficiency in the presence or absence of added azaguanine. The results are discussed as a possible confirmation of the proposed antimutagenic action of quinacrine hydrochloride on mammalian cells.

¹ Present address: Department of Microbiology, University of Michigan, School of Medicine, Ann Arbor, Michigan.

² All correspondence should be addressed to this author.

Received 11/18/68. Accepted 3/18/69.