This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Zajac, B. A. Articles by Henle, G. Search for Related Content PubMed PubMed Citation Articles by Zajac, B. A. Articles by Henle, G.

Autogenous and Virus-induced Interferons from Lines of Lymphoblastoid Cells¹

Virus Laboratories of the Children's Hospital of Philadelphia and School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19146

Lymphoblastoid cell lines derived from Burkitt's lymphoma or leukocytes of leukemic or healthy donors were examined for their ability to produce autogenous and virus-induced interferons. All interferon yields were maximal when 10⁷ cells/ml were incubated for 24 hr on a rotary shaker at 37°C. Of 10 viruses tested only Newcastle disease virus (NDV) and reovirus type 3 were found to be effective inducers of interferon. The cell lines differed with respect to (a) the yields of autogenous interferon (0 to ã32 units/ml); (b) the yields of virus-induced interferon (8–1024 units/ml); and (c) the minimal input multiplicities of active or UV-inactivated NDV (1–50) required to induce detectable interferon synthesis. Maximal yields of interferon were obtained in all instances with NDV or NDV_uv at an input multiplicity of 50.

Autogenous and virus-induced interferons could not be differentiated by various chemical or physical procedures. By means of gel filtration, using Sephadex G-100, the molecular weights were estimated to be about 26,000 for 2 autogenous and 2 virus-induced interferons. While cultures yielding autogenous interferons may nevertheless be highly susceptible to vesicular stomatitis virus (VSV), they could be protected by addition of concentrated autogenous interferon derived from autochthonous cells. The protection was, however, less enduring than that conferred with virus-induced interferon. Results obtained with 16 cloned lines of Ogun cells suggested that all cells of this line produce small amounts of autogenous interferon rather than that a few cells synthesize large quantities. Kinetic studies revealed that the presence of a critical amount of autogenous interferon enhanced the speed of virus-induced interferon synthesis. Peak titers were obtained within 6–8 hr under these conditions, whereas a lag phase of 8 hr was observed in lines free of autogenous interferon, and maximal yields were not attained before 17 hr after viral stimulation. Puromycin blocked both virus-induced and autogenous interferon production. The factor responsible for autogenous interferon synthesis remains obscure.

¹ Supported by Grants CA-04568 and 5T1-AI-00104, NIH, USPHS.

² Career Award 5-K6-AI-22,683, NIH, USPHS.

Received 12/17/68. Accepted 3/31/69.

This article has been cited by other articles:

				J. De Maeyer-Guignard Mouse Leukemia: Depression of Serum Interferon Production Science, September 1, 1972; 177(4051): 797 - 799. [Abstract] [PDF]