This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Rusconi, A. Articles by DiMarco, A. Search for Related Content PubMed PubMed Citation Articles by Rusconi, A. Articles by DiMarco, A.

Inhibition of Nucleic Acid Synthesis by Daunomycin and its Relationship to the Uptake of the Drug in HeLa Cells

Istituto Nazionale per lo Studio e la Cura dei Tumori, Sezione di Chemioterapia Sperimentale, 20133 Milano, Italy

Some aspects of the action of Daunomycin on nucleic acid synthesis was investigated in HeLa cells. Increasing concentration of the drug caused a proportionally higher inhibition of the incorporation of nucleic acid precursors. The relative inhibition of uridine-³H and thymidine-³H incorporation appeared to be dependent on the concentration of the drug in the medium and on the time of contact with the cell. The inhibition caused by Daunomycin was also found to be related to the cell population density.

The uptake of the antibiotic by HeLa cells was studied by means of tritiated Daunomycin. The uptake was rapid and nearly complete within 20 minutes. The amount fixed per cell seemed to be dependent only on the drug concentration in the medium. A quantitative relationship between the inhibition of uridine-³ H incorporation and the uptake of the antibiotic was demonstrated.

Received 10/30/68. Accepted 3/28/69.

This article has been cited by other articles:

				T. Triton and G Yee The anticancer agent adriamycin can be actively cytotoxic without entering cells Science, July 16, 1982; 217(4556): 248 - 250. [Abstract] [PDF]