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Separation of DNA Polymerase from Rat Liver and Hepatomas^1,2,

Duke University Medical Center and Veterans Administration Hospital, Durham, North Carolina 22705

Sephadex G-200 column chromatography of hepatoma DNA polymerase yields two peaks of enzyme activity. Peak I contains enzyme having a marked preference for denatured DNA, and the levels of this enzyme increase in proportion to tumor growth rate. Peak II contains the enzyme fraction having a moderate preference for native DNA. Normal and regenerating rat liver have a predominance of Peak II, in contrast to hepatomas. Peak I has been described as the major peak in fetal rat liver, and the present results are discussed in terms of repression of Peak I in adult liver and derepression which occurs as part of the process of malignant transformation.

¹ This is the third paper of a series entitled "DNA Replication and Degradation in Mammalian Tissues."

² Supported in part by grants from the USPHS (RO-CA-08800-02) and the American Cancer Society (P363C).

Received 11/29/68. Accepted 4/24/69.

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				E. F. Baril, M. D. Jenkins, O. E. Brown, and J. Laszlo DNA Polymerase Activities Associated with Smooth Membranes and Ribosomes from Rat Liver and Hepatoma Cytoplasm Science, July 3, 1970; 169(3940): 87 - 89. [Abstract] [PDF]