This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Fogh, J. Search for Related Content PubMed PubMed Citation Articles by Fogh, J.

Reduced Tumor-producing Capacity of Mycoplasma-modified Lines of FL Human Amnion Cells in the Cheek Pouch of Cortisonized Hamsters¹

Division of Experimental Pathology, Sloan-Kettering Institute for Cancer Research, New York, New York 10021

Long-term mycoplasma (Strain HT) infection of cells of the FL line of human amnion has distinctly reduced their tumor-producing capacity in the cheek pouch of cortisonized weanling golden Syrian hamsters. The number of cells necessary for tumor production (TPD₅₀) was increased in the order 5 to 10 times, as compared to uninfected FL cells, for three different cell lines originating from independent experiments (F 138, F 159, HTP8). An apparent increase in average tumor volume observed for these mycoplasma-infected cell lines was not an expression of high rate of growth of the tumor cells but was the result of the concurrent infection. After elimination of mycoplasma from the cell lines in vitro (F 138cl, F 159cl, HTP8cl), the change in TPD₅₀ was maintained, even after one year; the size of tumor resulting from a certain inoculum was reduced, and regression occurred earlier in comparison with the results obtained with the uninfected FL cells. Correlated with the previously reported changes in the in vitro behavior and chromosomal constitution of these mycoplasma-modified cell lines, the present in vivo results add further support to the degrading of the malignant state of FL human amnion cells accomplished by long-term mycoplasma infection.

¹ This investigation was supported in part by National Cancer Institute Grant No. CA-08748.

Received 7/ 3/68. Accepted 5/12/69.