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Benzoylated Diethylaminoethyl Cellulose Chromatography of Tumor and Nontumor Transfer RNA¹

Department of Microbiology, Indiana University, Bloomington, Indiana 47401

tRNA isolated from rabbit liver, Ehrlich ascites tumor cells, and L-cells was chromatographed on benzoylated diethylaminoethyl cellulose. With nonacylated tRNA, it was possible to resolve three species of tyrosyl-tRNA, four or five species of lysyl-tRNA, and five species of seryl-tRNA. The elution patterns of acylated tRNA's were also examined. Two isoaccepting phenylalanyl-tRNA's were resolved. When acylated tRNA's from these sources were cochromatographed, no qualitative differences were detectable, although quantitative differences were present.

¹ This work was supported in part by USPHS Grants CA-10417 and CA-11496 and a grant from the Damon Runyon Memorial Foundation, DRG-1018.

² The abbreviations used are: MAK, methylated albumin-Kieselguhr; BD, benzoylated diethylaminoethyl cellulose.

Received 4/ 3/70. Accepted 6/10/70.