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In Vitro Cultivation and Antigenicity of Cottontail Rabbit Papilloma Cells Induced by the Shope Papilloma Virus¹

Laboratory of Viral Oncology, Research Institute, Aichi Cancer Center, Chikusa-ku, Nagoya, Japan

The cells from a cutaneous papilloma of the cottontail rabbit induced by the Shope papilloma virus (SPV) were cultured in vitro, and three continuously growing cell lines, designated as CRSP-1, CRSP-2, and CRSP-3, were established. A continuously growing cell line designated as NCR-1 was also established from the normal skin of a cottontail rabbit. The cell lines including NCR-1 consist of mostly spindle-shaped and a few polygonal cells. The viral antigen specific for SPV was demonstrated in the cytoplasms of CRSP-1, CRSP-2, and CRSP-3 cells by immunofluorescent staining. However, under electron microscopic observation, SPV virions were not detectable in the cytoplasms or nuclei. On the other hand, tumor-specific T-antigen and surface antigen were clearly demonstrated in the nuclei and on the cell membrane of the cells of CRSP cell lines, respectively. The CRSP-1 cells were inoculated into domestic rabbits to examine whether the cells retain capacity to produce infectious SPV. No papillomatous growth or tumorous development was seen at the site of the inoculation in any rabbits.

¹ This work was supported in part by Grants from the Jane Coffin Childs Memorial Fund for Medical Research and from Eli Lilly and Company.

Received 4/24/70. Accepted 7/ 1/70.