This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Chernozemski, I. N. Articles by Warwick, G. P. Search for Related Content PubMed PubMed Citation Articles by Chernozemski, I. N. Articles by Warwick, G. P.

Liver Regeneration and Induction of Hepatomas in B6AF₁ Mice by Urethan¹

Chester Beatty Research Institute, Institute of Cancer Research, Royal Cancer Hospital, Fulham Road, London S. W. 3, England

The pattern of regeneration and cell proliferation was studied in liver remnants of B6AF₁ hybrid mice during the first 85 hr following partial hepatectomy. On the basis of the estimated timing of DNA synthesis and mitoses, male and female adult mice were inoculated once with urethan, 1 mg/g body weight, at the following times after partial hepatectomy: 4 to 5 hr, i.e., at an early prereplicative period; 17 to 18 hr, during a late prereplicative period; 31 to 33 hr, shortly before the onset of extensive DNA duplication; and 46 hr, before the peak of mitoses.

The hepatoma incidence showed a clear-cut relationship between the timing of urethan treatment in relation to prior hepatectomy. After 13 months, 10, 68, 42, and 77% of the male animals bore at least one hepatoma, compared with 3.3% among untreated males, 6% in those treated only with urethan, and 19.4% following hepatectomy alone. Hepatic tumors were rare in the females. In urethan-treated mice, body weight was reduced by up to 50% of that of controls.

¹ This study was supported by a grant from the Medical Research Council and British Empire Cancer Campaign for Research.

² This work was carried out during a Research Fellowship of the International Agency for Research on Cancer, Lyon, France, while on leave from Oncological Research Institute, Sofia 56, Bulgaria.

³ To whom reprint requests should be addressed at Chester Beatty Research Institute.

Received 3/27/70. Accepted 7/14/70.