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The Preparation of Insoluble, Matrix-supported Derivatives of Asparaginase for Use in Cancer Therapy¹

Biochemistry Section, Cancer Research Center, Columbia, Missouri 65201, and Travenol Laboratories, Inc., Morton Grove, Illinois 60053

Four methods are described for the preparation of enzymically active, water-insoluble derivatives of the antineoplastic enzyme asparaginase from Escherichia coli B. All methods lead to covalent bond formation, and three of the derivatives are more stable at 37° than free enzyme in solution. Derivatives prepared by the Woodward's reagent method are slightly less stable than free enzyme at 37°. The materials are suggested for use in an extracorporeal filter.

¹ This work was supported by Travenol Division, Baxter Laboratories, Morton Grove, Ill.

Received 6/ 4/70. Accepted 7/28/70.