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Difference in Electrokinetic Charge of Cells between Two Cell Types of Ascites Hepatoma after Removal of Sialic Acid¹

Laboratory of Cell Biology, Aichi Cancer Center Research Institute, Nagoya, Japan

Comparative studies on the effect of enzyme digestion of sialic acid on the cellular electrokinetic charge were performed on two types of rat ascites hepatoma.

There was no detectable change in the electrokinetic charge in the enzyme-treated cells from island-forming lines, whereas the negative surface charge was remarkably reduced in the treated cells from the free-cell lines. This difference in the electrokinetic property as detected by the enzyme treatment was simply dependent upon the surface property of cells, island-forming or free-living, which was cytogenetically fixed.

Electron microscopic observations and assay of released sialic acid revealed that sialic acid was similarly removable by the enzyme treatment from the cell surface in both types.

These results are discussed in connection with the chemical nature of the cell surface in both cell types.

¹ This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education in Japan.

² Present address: Department of Pathology, Nagoya City University Medical School, Nagoya, Japan.

Received 1/26/70. Accepted 8/10/70.