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Incidence of EB Virus-containing Cells in Primary and Secondary Clones of Several Burkitt Lymphoma Cell Lines¹

Department of Viral Oncology and The Cell Laboratory, Roswell Park Memorial Institute, Buffalo, New York 14203

Several Burkitt lymphoma cell lines were cloned by two procedures: (a) an agar droplet method and (b) a single-cell isolation procedure. The derived clones were examined for the presence of Epstein-Barr virus containing cells by an indirect immunofluorescent antibody test. The clones were established so as to ensure that single virion-negative cells initiated the clones. This included the microscopic selection of single cells, the use of Epstein-Barr virion-combining human 7S globulin, and a cell washing procedure the total dilution of which greatly exceeded the highest reported concentration of extracellular, enveloped particles. Ninety-two to 100% of the clones established from the high-incidence group of virus-containing parental cell lines were virus positive, whereas 7 to 23% of the clones established from the low-incidence group of virus containing parental cell lines were virus positive. Recloning indicated that negative clones, found in either group, could give rise to virus-positive secondary clones. The data reported here support the hypothesis that all Burkitt lymphoma derived cells carry a hereditary potential for Epstein-Barr virus production and that the rate at which this potential is induced determines the virus positiveness of that particular cell line.

¹ Supported in part by United Health Foundation Grant GR-11-RP-69.

² Present address: Second Department of Surgery, Nagoya University School of Medicine, Nagoya, Japan.

Received 7/ 1/70. Accepted 8/24/70.

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