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Studies on Liver Plasma Membranes of Rats Fed N-2-Fluorenylacetamide¹

The Burnsides Research Laboratory, University of Illinois, Urbana, Illinois 61801

Plasma membranes were prepared from livers of normal and N-2-fluorenylacetamide-ingesting rats. Of the two marker enzymes studied, the activity of 5'-nucleotidase was markedly decreased, whereas that of Mg²⁺-Na⁺-K⁺-ATPase was unaffected in the membranes from animals fed the carcinogen. The percentage of phospholipids in the preneoplastic and neoplastic liver membranes was very much higher than in the controls, and the phospholipid:cholesterol ratio was also significantly enhanced. The percentage of polyunsaturated fatty acids was higher in these membranes than in the plasma membranes of normal liver. Unlike in preparations from normal livers, a large amount of floating fraction (d < 1.16) was invariably observed in membrane preparations from preneoplastic and neoplastic livers.

¹ Supported by a grant from the American Cancer Society and Grant CA-01932 from the USPHS. A preliminary report has been published (6).

² On leave from the Biochemistry Division, Central Food Technological Research Institute, Mysore, India.

³ Recipient of a Research Career Development Award, 5K3-CA-31,063 from the National Cancer Institute.

Received 6/29/70. Accepted 8/21/70.