This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Schlede, E. Articles by Conney, A. H. Search for Related Content PubMed PubMed Citation Articles by Schlede, E. Articles by Conney, A. H.

Stimulatory Effect of Benzo()pyrene and Phenobarbital Pretreatment on the Biliary Excretion of Benzo()pyrene Metabolites in the Rat¹

The Wellcome Research Laboratories, Burroughs Wellcome & Co. (U. S. A.) Inc., Tuckahoe, New York 10707

Benzo()pyrene (BP) metabolites are excreted into the bile at a slower rate after an i.v. injection of ¹⁴C-labeled BP than after an injection of ¹⁴C-labeled biliary metabolites of BP. Pretreatment of rats with BP or phenobarbital prior to the i.v. injection of 10 or 300 µg of BP-¹⁴C enhances the rate of excretion of metabolites of BP-¹⁴C into the bile. No effect of pretreatment was observed when ¹⁴C-labeled metabolites of BP, instead of BP-¹⁴C, were injected i.v., which indicates that pretreatment with BP or phenobarbital does not enhance the transport of BP metabolites into bile but enhances the formation of BP metabolites. The results described in this manuscript and elsewhere (14) indicate that pretreatment of rats with BP enhances the in vivo hydroxylation of BP-¹⁴C, whereas phenobarbital does not exert this effect. The data suggest that treatment of rats with phenobarbital prior to the administration of BP-¹⁴C may enhance the biliary excretion of BP-¹⁴C metabolites by enhancing the conjugation of hydroxylated BP metabolites.

¹ Supported in part by Research Contract PH 43-65-1066 from the Pharmacology-Toxicology Programs, National Institute of General Medical Sciences, NIH.

² Present address: Pharmakologisches Institut der Freien Universität, (Abt. Embryonal-Pharmakologie), 1 Berlin-Dahlem (33), Thielallee 69/73, West Germany.

³ Present address: Department of Biochemistry, Hoffmann-La Roche, Inc., Nutley, N. J. 07110.

Received 5/ 1/70. Accepted 8/19/70.

This article has been cited by other articles:

				A. H. Conney Enzyme Induction and Dietary Chemicals as Approaches to Cancer Chemoprevention: The Seventh DeWitt S. Goodman Lecture Cancer Res., November 1, 2003; 63(21): 7005 - 7031. [Abstract] [Full Text] [PDF]