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A Study of Surface Ionogenic Groups of Different Types of Normal and Leukemic Cells¹

Department of Experimental Medicine and Cancer Research, Hebrew University, Hadassah Medical School, Jerusalem, Israel

The electrophoretic properties of normal lymphoid cells and macrophages were compared to those of leukemic cells obtained from the C58, AKR, and SJL/J strains. Mobilities were analyzed as to their relationship to pH, affinity to calcium, and susceptibility to neuraminidase. No major differences in overall pattern of electrokinetic behavior have been detected between the malignant cells and the normal cells. Each type of cell, however, exhibited its own characteristic surface charge properties, the lymph node and spleen cells being of slightly higher mobility values than the thymocytes and macrophages. The lymph node cell was distinguished from the other cells by its pronounced heterogeneity in all the parameters tested. Neuraminidase reduced the mobility of all the cells tested by about 20 to 40%. The malignant cells did not generally show increased surface charge density or a decreased affinity to calcium as compared to their normal counterparts. The results are discussed in relation to the hypothesis that the malignancy of cells is correlated with decreased adhesiveness, decreased calcium-binding power, and increased charge density.

¹ This investigation was supported by USPHS Grant CA-06582 from the National Cancer Institute.

Received 3/21/69. Accepted 6/26/69.