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A Study of Surface Ionogenic Groups of Chick Embryo Cells Transformed by Rous Sarcoma Virus

Department of Experimental Medicine and Cancer Research, Hebrew University, Hadassah Medical School, Jerusalem, Israel

The electrokinetic surface properties of chick embryo cell cultures transformed by Rous sarcoma virus have been studied for any alterations associated with the malignant transformation. In this system a rapid and efficient transformation in vitro takes place which enables a comparison to be made between normal and malignant cells derived from the same embryo and cultured under the same conditions. No difference could be detected between the normal and malignant cells for either the surface charge or pH-mobility relationship. The expected decrease in calcium-binding power of the malignant cells was not evidenced in this system, as both types of cells also had the same affinity to calcium. These data as well as other recent studies suggest that the relationship between calcium affinity and malignancy is in need of further clarification.

Neuraminidase treatment produced a mobility decrease in malignant cells that never surpassed that noted in normal cells but was always either equivalent to or less than that of their normal counterparts. It was concluded that no generalization could be made as to the correlation between malignancy, increased surface charge density, and increased surface sialic acid.

Received 3/21/69. Accepted 6/26/69.