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Quantitative Aspects of Thymidine Uptake into the Acid-soluble Pool of Normal and Polyoma-transformed Hamster Cells¹

Department of Microbiology, The University of Rochester, School of Medicine and Dentistry, and Strong Memorial Hospital, Rochester, New York 14620

The uptake of thymidine into an acid-soluble pool in normal hamster embryo cells and hamster tumor cells transformed by polyoma virus was studied. It was found that tumor cells exhibited a 4-fold higher capacity than normal cells to incorporate thymidine as phosphorylated products into this pool. This was correlated with a 5- to 10-fold higher activity of thymidine kinase in the tumor cell lines. The uptake process was strongly inhibited by the thymidine analog iododeoxyuridine, partially inhibited by deoxyuridine, and not inhibited by uridine.

¹ Supported in part by NIH Grants CA-07239 and CA-05206.

² Recipient of Research Career Development Award AIK3-13959.

Received 5/ 5/69. Accepted 8/27/69.