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Studies on an Acid Ribonuclease from Corticoid-sensitive 9-Fluoroprednisolone-treated Lymphosarcoma P1798: Changes in Heat Inactivation Kinetics of the Enzyme due to Steroid Treatment¹

Research Institute of the Hospital for Joint Diseases, Mt. Sinai School of Medicine, New York, New York 10035

An acid ribonuclease has been purified from corticoid-sensitive lymphosarcoma P1798 18 hr after a single injection of 9-fluoroprednisolone into host mice, and the properties of the enzyme were studied. This enzyme differs in heat inactivation kinetics from the acid ribonuclease of control tumors purified by identical methods. The enzymes from control and steroid-treated tumors do not show differences in sedimentation behavior, isoelectric point, K_M, and specificity towards substrates.

¹ This work was supported by Contract PH 43-64-46 from the Endocrine Evaluation Branch, General Laboratories and Clinics, National Cancer Institute.

Received 7/22/69. Accepted 8/28/69.