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Productive and Abortive Growth of an Oncogenic Simian Adenovirus SV20 in Cultured Cells¹

Section of Laboratory Medicine, Yale University, School of Medicine, New Haven, Connecticut 06510, and Veterans Administration Hospital, West Haven, Connecticut 06516

The growth characteristics of an oncogenic simian adenovirus, SV20, in rhesus monkey (RhMK) and hamster kidney (HamK) cell cultures were compared. The adsorption rate of SV20 in both cell systems was rather slow, but increased steadily up to 6 hr. There was 3 to 8 times more cell-associated virus in RhMK cells than in HamK cells during the first 6 hr of infection. A complete growth cycle of SV20 was obtained in infected monkey cells. This includes sequential nuclear alterations, production of T antigen, viral antigen, and infectious virus. In hamster cells, SV20 induced an abortive infection. Eosinophilic inclusions and T antigen were observed as two distinct entities in the infected hamster cells, but no viral antigen or infectious virus was found in the hamster cell system. Marked stimulation of DNA synthesis in SV20-infected hamster cells was observed.

¹ This investigation was supported by USPHS Research Grant AI-08648 from the National Institute of Allergy and Infectious Diseases and Veterans Administration Research Funds.

² Present address: Putnam Memorial Hospital Institute for Medical Research, Bennington, Vt. 05201.

Received 2/27/69. Accepted 8/27/69.