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Detection of a Reactive Intermediate in the Reaction between DNA and Hydroxyurea¹

Department of Microbiology, College of Physicians and Surgeons, Columbia University, New York, New York 10032

Exposure of DNA to hydroxyurea resulted in degradation of the polydeoxynucleotide. This reaction is dependent upon time, temperature, and pH and has been associated with the transformation of hydroxyurea to a reactive derivative. Addition of the reactive derivative to DNA resulted in rapid degradation. Preliminary findings suggest that N-carbamoyloxyurea is the transformation product in the reactive derivative responsible for DNA degradation.

¹ This investigation was aided by Contract Nonr 266(89) NR-136-574 between the Office of Naval Research, Department of the Navy, and Columbia University. Support was also provided by a grant from the National Cancer Institute, USPHS and by the George Alexander Carden, Jr., Special Fund for Cancer Research.

² Present address: Department of Microbiology and Immunology, College of Medicine, Downstate Medical Center, Brooklyn, N. Y. 11203.

³ Research Career Development Awardee (5-K 3-GM29,024) of the Division of General Medical Sciences, USPHS.

Received 6/26/69. Accepted 11/ 3/69.