This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Sobin, L. H. Search for Related Content PubMed PubMed Citation Articles by Sobin, L. H.

High-Resolution Autoradiographic Localization of 3,4-Benzpyrene-³H in Mouse Skin

Department of Pathology, New York Hospital-Cornell Medical Center, New York, New York 10021

Tritiated 3,4-benzpyrene was traced at intervals between 1 hr and 21 days by autoradiography with both light and electron microscopes after topical application to the skin of mice. The sebaceous glands were the most heavily labeled site 1 and 4 hr after application. Electron microscopy showed labeling of a variety of cellular components in both sebaceous and epidermal cells. No one organelle appeared to be exclusively associated with the tracer. Nuclei were labeled in proportion to their cytoplasm, but generally to lesser degrees. The findings are consistent with previous work on the binding of hydrocarbon carcinogens to soluble proteins and DNA. The data also support the hypothesis that the binding of carcinogen is proportional to cellular synthetic activity.

¹ Present address: Cancer Unit, World Health Organization, 1211 Geneva 27, Switzerland.

Received 8/25/69. Accepted 10/23/69.