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Nucleolinar Morphology in Normal Diploid, Neoplastic, and Aneuploid Cells in Vitro¹

Department of Pathology, Jefferson Medical College, Philadelphia, Pennsylvania 19107

The relationship between nucleolar morphology and karyotype has been examined under varying conditions of tissue culture. Studies of nucleolar morphology have been extended to 5 more diploid human lines and 6 more aneuploid lines. As previously reported (4, 5), the nucleolini of diploid cells were uniform in size within the same nucleus (isonucleolinosis), while nucleolini of aneuploid cells varied considerably in size within a given nucleus (anisonucleolinosis) under optimal nutritional conditions. After prolonged culture without replenishment of medium, diploid cells became anisonucleolinar. Isonucleolinosis could be restored by replacement of medium although eventually when the cultures became several layers thick the cells again became anisonucleolinar. Evidence is presented to indicate that nutritional factors rather than density-dependent inhibition of replication were responsible for the change in nucleolinar morphology. Aneuploid cells were anisonucleolinar under all conditions of growth although the disparity in nucleolinar size increased during culture, especially when the medium was not replaced. A study of various passage levels of mouse embryo cells indicated that the cells became aneuploid and anisonucleolinar at all stages of culture after 37 passages. Anisonucleolinar cells from the 41st passage produced fibrosarcomas when inoculated into syngeneic mice.

¹ Supported by NIH Grant CA-05402-10.

Received 8/ 1/69. Accepted 9/26/69.

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