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Adenocarcinoma of Glandular Stomach and Duodenum in Wistar Rats Ingesting N-Methyl-N'-nitro-N-nitrosoguanidine, Histopathology and Associated Secretory Changes¹

Temple University School of Medicine, Philadelphia, Pennsylvania 19140

N-Methyl-N'-nitro-N-nitrosoguanidine (NG) produces carcinoma of the glandular stomach and duodenum in rats when administered continuously in drinking water at a concentration of 83 mg/liter. The earliest histological changes consist of submucosal edema and basal mucosal fibrosis and were seen by the 3rd day on NG. By the 41st week on NG, 60% of rats develop tumors and by the 52nd week 80% of the animals had gastric adenocarcinoma, mostly in the antrum.

Changes in gastric secretion were evaluated by the 4-hr pylorus ligation technique. Volume of gastric secretion and titratable acidity remained at control levels until late in the course of mucosal atrophy and cancer development. Pepsin secretion increased initially but gradually returned to control levels by the end of the year of NG exposure.

Both the high frequency of tumor formation and the minimal toxicity of NG suggest that this carcinogen may be ideal for use in an experimental model studying gastric carcinogenesis.

¹ Supported in part by NIH Research Grant CA-10439, USPHS, and by Fels Research Institute. Temple University School of Medicine.

² Present address: Professor of Medicine, Jefferson Medical College, Thomas Jefferson University, 1025 Walnut Street, Philadelphia, Pa. 19107.

Received 9/ 3/69. Accepted 10/16/69.