This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Black, D. D. Articles by Griffin, A. C. Search for Related Content PubMed PubMed Citation Articles by Black, D. D. Articles by Griffin, A. C.

Similarity of the Transfer Factors in Novikoff Ascites Tumor and Other Amino Acid-incorporating Systems¹

Department of Biochemistry, The University of Texas, M. D. Anderson Hospital and Tumor Institute at Houston, Houston, Texas 77025

A highly active in vitro amino acid-polymerizing system has been isolated from Novikoff ascites tumor cells. Considerable difficulty was encountered in the resolution of the transfer enzyme complex. However, purified ribosomes and the transfer components were prepared, and it was possible to demonstrate that 2 factors are involved in the polymerization of amino acids by this tumor cell system. The 2 factors were interchangeable with components of the rabbit reticulocyte system. Other investigators have shown that the 2 transfer factors of the rat liver and reticulocyte systems are interchangeable. These findings suggest that the polysomal mechanism of protein synthesis is quite similar in all mammalian cells and possibly in all cells. However, the ribosomes and Factor T₂ or G of the mammalian and microbial systems are not interchangeable.

¹ Supported by American Cancer Society Grant P-501D and the Robert A. Welch Foundation Grant G-035.

² American Cancer Society Professor of Biochemistry.

Received 9/ 8/69. Accepted 11/19/69.