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Relationship of Cx-reactive Protein Response to Induction of Liver and Kidney Catalases following Hematin Administration into Rabbits

Department of Pathology, University of Hawaii School of Medicine, Honolulu, Hawaii 96822, and the Leahi Hospital, Honolulu, Hawaii 96816

The relationship of rabbit Cx-reactive protein (CxRP) and the enzymatic activity of hepatic and kidney catalases in rabbits following i.v. hematin administration has been examined sequentially. The early increases in rabbit liver and kidney catalatic activities were accompanied by the corresponding decreases in ß CxRP and an early appearance of CxRP after hematin administration. Immunoelectrophoretic analysis of serum samples revealed two types of CxRP: a preexisting, although variable in concentration, ß CxRP and a CxRP which appeared within 3 hr and then disappeared after 24 hr following a single injection of hematin. Adjuvant-treated rabbits showed slight depression of liver catalase with an increase in ß and CxRP levels. When hematin was administered to these animals, a rise in both liver and kidney catalases ensued with a concomitant decrease in serum ß and CxRP's 3 hr after hematin. Actinomycin D. Nogalamycin, and puromycin had essentially no effect on the synthesis of liver catalase in tissue slice culture studies. Purified C-reactive protein interacted with hematin to give variable peroxidatic activity. The data presented suggest the presence of protein precursor pools for the formation of hemoproteins and that, in part, these may be CxRP. Thus, any measure of CxRP or C-reactive protein in pathological situations may be a reflection of changes in the metabolic events of the oxidative system, specifically those of catalases.

¹ This investigation was supported in part by USPHS Research Grant R01 CA 10671-02 from the NIH.

Received 6/23/69. Accepted 12/ 4/69.